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YouTubethe bumbling biochemist
Fast performance liquid chromatography (FPLC) used in research labs to purify recombinant proteins.
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Keep a log of your FPLC (e.g. AKTA) runs, as well as screenshots
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Lab tip: Set aside & save some buffer
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Brianna Bibel on Instagram: "The keys to connecting a SEC (Size Exclusion Chromatography)/Gel Filtration column to an FPLC: don't let in air; don't let the column get dry; don't let the pressure get too high. The key steps: 1) prime and wash the pump 2) go to column handling to find the pre-column pressure limit for the column you're using 3) go to manual → execute manual instructions and set that pre-column pressure limit (5.0 for a Superdex 200 10/300 GL) 4) set the column position to the colu
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Brianna Bibel on Instagram: "The keys to connecting a SEC (Size Exclusion Chromatography)/Gel Filtration column to an FPLC: don't let in air; don't let the column get dry; don't let the pressure get too high. The key steps: 1) prime and wash the pump 2) go to column handling to find the pre-column pressure limit for the column you're using 3) go to manual → execute manual instructions and set that pre-column pressure limit (5.0 for a Superdex 200 10/300 GL) 4) set the column position to the colu
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Instagramthebumblingbiochemist
Got an AKTA loop valve :)
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Got an AKTA loop valve :)
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