By the early 1950s, most clinical microbiology laboratories in the United States had adopted the disk diffusion method for determining susceptibility of bacteria to antimicrobials.

In diagnostic laboratories, the test is performed by inoculating the surface of an agar plate with bacteria isolated from a patient's infection. Antibiotic-containing paper disks are then applied to the agar and …

Jan 1, 2013 · Disk diffusion is a conventional phenotypic method for antimicrobial susceptibility testing that combines simplicity and cost-effectiveness.

Jul 27, 2025 · The disk diffusion assay is a laboratory procedure designed to evaluate how effectively antimicrobial agents inhibit bacterial growth. This technique is widely used to determine if a specific …

The disc diffusion method is a common and routine method employed in clinical laboratories. In this approach, small filter paper disks that have been soaked in a known quantity of antibiotic are …

Jan 5, 2026 · The Kirby Bauer Disc Diffusion Method is commonly used in clinical microbiology laboratories to determine the susceptibility or resistance of bacteria to various antimicrobial agents.

May 18, 2022 · Kirby Bauer tests also known as the Disc diffusion test is used for antibiotic susceptibility testing. In 1961, WHO standardized the procedure.

Using a disk dispenser or sterile forceps, the disks are placed in even array on the plate, at well-spaced intervals from each other. When the disks are in firm contact with the agar, the antimicrobial agents …

The disk diffusion method is performed using Mueller-Hinton Agar (MHA), which is the best medium for routine susceptibility tests because it has good reproducibility, low in sulfonamide, trimethoprim, and …

Jun 13, 2025 · Disk diffusion is a widely used method in microbiology for determining the antimicrobial susceptibility of bacteria. The technique involves placing a paper disk impregnated with a known …